Journal of Biotech Research & Biochemistry Category: Biotechnology Type: Research Article

Effect of Dietary Supplementation of Cymbopogon Citratus Oil On The Haematology And Serum Biochemical Parameters Of Broiler Chicks

Alagbe JO1*
1 Department Of Animal Nutrition And Biochemistry, Sumitra Research Institute, Gujarat, India

*Corresponding Author(s):
Alagbe JO
Department Of Animal Nutrition And Biochemistry, Sumitra Research Institute, Gujarat, India

Received Date: Nov 06, 2020
Accepted Date: Nov 16, 2020
Published Date: Nov 23, 2020


This study was carried out to investigate the effects of dietary supplementation of Cymbopogoncitratus oil (LGO) on the haematology and some serum biochemical parameters of broiler chicks. 250-1 day old broiler chicks of Ross 308 strain were randomly divided into five (5) dietary treatments of 5 replicates and each replicates had ten (10) birds. Birds in treatment 1 (T1) were fed basal diet with 0 % LGO, T2, T3, T4 and T5 were fed diet supplemented with LGO at 0.1 %, 0.2 %, 0.3 % and 0.4 % respectively in a completely randomized design. Basal diet was formulated to meet the nutritional requirement of birds according to NRC (1994), feed and water were given ad libitum, all necessary management practices were strictly observed throughout the experiment which lasted for 56 days. Data collected revealed that haemotological parameters (PCV, Hb, RBC, MCV, MCH, MCHC, WBC and its differentials) and serum biochemical analysis (total protein, albumin, globulin, cholesterol, creatinine, uric acid, LDL, HDL, triglycerides, ALT, AST and ALP)were significantly affected (P<0.05) by the dietary supplementation of LGO. Serum electrolytes (sodium, chloride, calcium and potassium) were not significantly (P > 0.05) influenced by the different inclusions of LGO. However, all values reported were within the normal range for healthy birds. It was concluded that LGO is loaded with various bioactive chemicals which possess significant biological and pharmacological activities, they are safe, effective and can be used to bridge the gap between food safety and production without causing any negative effect on the health and general performance of birds.


Broiler chicken; Cymbopogon citratus oil; Food safety; Haematology; Serum electrolytes


Due to challenges of antibiotic resistant bacteria, higher cost, anticipated toxicity and dangers posed to human health, there is growing awareness on the use of natural medicines which are more relatively effective, cheap and safe in prolonged use [1-3]. Among the potential natural alternatives is the use of Essential Oil (EOs); essential oil derived from plants have provided enough evidences to suggest as a tool for defending bacteria diseases, regulate feed intake and secrete digestive secretions [4]. They are also loaded with several bioactive chemicals or phytochemicals (alkaloids, flavonoids, saponins, terpenoids, phenols, tannins etc.) which performs a wide range of pharmacological activities such as: antimicrobial, antiviral, antioxidant, hypolipidemic, antifungal, hepato-protective, neuro-protective, antispasmodic, anti-allergic, immunomodulatory and hypotensive properties without showing any negative effects in especially oil from lemon grass (Cympobogoncitratus) [5-10]. According to EOs are volatile substances of a complex mixture of chemicals components which evaporate when in contact with air and are biosynthesized by plants. 

Cymbopogon citrates (Stapf) commonly referred to as lemon grass belongs to the family Poaceae. The plant is a tropical grass resistant to different temperature and can grow in warm, semi-warm and temperate climates [11]. Cymbopogon is derived from two greek words “Kymbe” meaning boat and “pogon” which means beard (arrangement of the spike of the flowers) while citratus is a latin word which means lemon-scented leaves [12,13]. Lemon grass is rich in various phytochemicals and is used in traditional system and folk medicine to treat malaria, pneumonia, gastrointestinal infections, anxiety and diabetes [14,15]. 

Previous studies have shown that EOs have been used as phytogenic feed additives to ensure balanced micro flora (eubiosis), scavenge free radicals or prevent oxidative stress, immune modulator and growth enhancer [16-19] there is also a correlation between nutrition (feeding) and immune system of an animal, therefore, proper feeding lessens the immune suppression associated with the stress response in the bird [20-21]. 

Therefore, in order to enhance food safety and explore other organic alternatives to antibiotics. This experiment was designed to examine the effect of dietary supplementation of Cymbopogoncitratus oil on the haematology and serum biochemical parameters of broiler chicks.

Materials and Methods

Study area 

The experiment was carried out at Division of Animal Nutrition, Sumitra Research Institute, Gujarat, India during the month of January to March, 2019. 

Collection, processing and extraction of lemon grass oil (LGO) Fresh, mature and healthy lemon grass (Cymbopogoncitratus) leaves were harvested within Sumitra Teaching and Research farm, Gujarat, India and identified by a plant taxonomist (Dr. Sharma Kumar), it was later washed with a running tap water to remove dirt’s and air dried for 15 days to maintain the bioactive chemicals in the plant and to prevent the growth of microorganisms until a constant was obtained, thereafter powdered and kept in an air tight well labeled container. Cymbopogoncitratus essential oil (LGO) was obtained according to the methods outlined. 

Pre-experimental operations 

Before the commencement of the experiment deep litter pens were properly disinfected, wooden planks are been used to demarcate each treatments and replicates, properly labeled, feed and water troughs were properly washed and foot bath was put in place to ensure proper biosecurity. 

Animals and their management 

Two hundred and fifty (250) one day old broiler chicks (Ross 308) strain weighing 45 ± 0.05 g/bird (mean ± SD) were purchased from a commercial hatchery in India. The birds were randomly assigned into five (5) treatments of 50 birds; each group was further sub-divided into 5 replicates of 10 birds. The experiment lasted for 56 days, vaccines were administered according to the prevailing vaccination schedule in the environment, feed and water were provided ad libitum and all necessary management practices were strictly observed. 

Formulation of experimental diet and design 

Three experimental diets (Basal diet) were formulated consisting of starter diet fed 0-21 days, growers mash (22-35 days) and finisher diet (36-56 days). Diets were formulated to meet the nutrition requirement of birds according to NRC (1994) as presented in (Table 1). The experiment was carried out in a Completely Randomized Design (CRD) and the set up goes thus: 

Treatment 1 (Control): Basal diet + 0 % LGO

Treatment 2: Basal diet + 0.1 % LGO

Treatment 3: Basal diet + 0.2 % LGO

Treatment 4: Basal diet + 0.3 % LGO

Treatment 5: Basal diet + 0.4 % LGO 


Starter (1-21 days)

Grower (22-35 days)

Finisher (36-56 days)





Wheat offal




Soya meal




Groundnut cake




Fish meal




Bone meal




























Determined analysis

Dry matter (%)




Crude protein (%)




Ether extracts (%)




Crude fibre (%)




Calcium (%)




Phosphorus (%)








Meth +Cyst




ME (Kcal/kg)




Table 1: Chemical composition of experimental dietsPremix supplied per kg diet: - vit A, 10,000 I.U; vit E, 5mg; vit D3, 3000I.U, vit K, 3mg; vit B2, 5.5mg; Niacin, 25mg; vit B12, 16mg; choline chloride, 120mg; Mn, 5.2mg; Zn, 25mg; Cu, 2.6g; folic acid, 2mg; Fe, 5g; pantothenic acid, 10mg; biotin, 30.5g; antioxidant, 56mg. 

Data collected 

Feed consumed was recorded daily while body weight was recorded weekly. Mortality was recorded as it occurs. 

Blood collection and analysis 

On the 56th day, three birds were randomly selected for haematological and serum biochemical parameters. 5 ml of blood samples were collected early in the morning around 7 am via the wing web of the birds into sample bottles containing Ethylene Diaminetetraacetic Acid (EDTA) as anticoagulant for heamatologicalstudies while another sample of blood was emptied into bottles free from EDTA for serum metabolites analysis, all the bottles collected were properly labeled and transferred to the laboratory for further analysis. Pack Cell Volume (PCV), Red Blood Cell (RBC), Hemoglobin (Hb) were determined according to methods outlined. White Blood Cell (WBC) and its differentials were analyzed using an automated machine (Sysmex, Model KU-209 HPT, India).Values of Mean Corpuscular Hemoglobin (MCH) Mean Corpuscular Volume (MCV) and Mean Corpuscular Hemoglobin concentration (MCHC) were calculated using the formula below:

MCV (fl) = PCV / RBC × 10

MCH (pg) = Hb/RBC × 10

MCHC (%) = Hb (100mg blood) / PCV × 100 

Total protein, glucose levels, creatinine, uric acid, Cholesterol, Triacylglycerols (TG), high density lipoproteins, activities of Alanine Transaminase (ALT), Aspartate Transaminase (AST), Alkaline Phosphatase (ALP), serum ions (sodium, chloride, calcium and potassium) were analyzed using commercial diagonistic kits (Humburg, .Braunschweig, Germany, Model- 3401- UI-MN09-A). Low Density Lipoprotein (LDL) was estimated using

LDL (mmol/L) = cholesterol - HDL- (TG/2.2). 

Caecal microbial population 

At the end of the experiment (56 days), caeca microbial count was conducted using 5 birds per treatments. A 10-fold serial dilution method, in which of 1% peptone solution was mixed with caeca samples and poured on Mac Conkey agar plates to determine the colony forming unit (cfu) of E. coli, lactobacilli and Salmonella typhi according to methods described [22]. 

Chemical analysis 

Proximate analysis of the experimental diet was carried out according to [23].

Statistical analysis

All data were subjected to one -way analysis of variance (ANOVA) using SPSS (23.0) and significant means were separated using Duncan multiple range tests [24]. Significant was declared if P ≤ 0.05.


Proximate composition of experimental diets 

The proximate composition of experimental diet is presented in (Table 1). The feeding was done in three phases (starter, growers and finisher mash).Starter mash (0-21 days) contains dry matter (DM) 90.10 %, crude protein (CP) 23.08 %, ether extract (EE) 5.03 %, crude fibre (CF) 3.06 %, calcium 0.98 %, phosphorus 0.47 % and energy value 2936.0 (Kcal/kg). The DM, CP, CF, EE, calcium, phosphorus and metabolisable energy of growers mash (22-35 days) contained 93.63 %, 20.11 %, 3.95 %, 4.28 %, 1.10 %, 0.51 % and 3000.8 Kcal/kg respectively. Finisher mash (36 -56 days) contained dry matter (92.04 %), CP (19.33 %), CF (3.42 %), EE (4.28 %), calcium (1.10 %), phosphorus (0.51 %) and metabolisable energy (3100.2 Kcal/kg). 

Haematological parameters of broiler chicken fed diets supplemented with LGO 

Table 2 contains the haematological traits of broiler chicken fed diet supplemented with different levels of LGO. The Pack Cell Volume (PCV), Heemoglobin (Hb), Red Blood Cell (RBC), Mean Corpuscular Haemoglobin (MCH), Mean Corpuscular Volume (MCV) and Mean Corpuscular Haemoglobin Concentration (MCHC) values ranged between 25.62-33.80 %, 7.55-11.89 g/dL, 1.94 – 2.50 (mm3 × 106), 132.1-153.9 fl and 29.46 - 35.44 g/dL respectively. WBC (19.41-28.11 mm3 × 106), lymphocytes 12.73- 8.55 (×103µl), monocytes 0.25-0.85 (×103µl) and neutrophils 18.20-29.00 (×103µl). All the parameters measured were significantly (P<0.05) different among the dietarytreatments and they follow similar trend. PCV, Hb, RBC, MCH, MCV, MCHC, WBC and its differentials (lymphocytes, monocytes and neutrophils) were highest in T3, T4 and T5, intermediate in T2 and lowest in T1 (P<0.05). 








PCV (%)







Hb (g/dL)







RBC (mm3 × 106)







MCV (fl)







MCH (pg)







MCHC (g/dL)







WBC(mm3 × 106)

Differentials (103µl)




























Table 2: Haematological parameters of broiler chicks fed different levels of LGO means in the same row with different superscripts differ significantly (P<0.05). 

SEM: Standard Error of Mean

The reduction in the serum electrolytes were not below the normal physiologic range reported for healthy birds. Drastic decrease will lead to anorexia, pyrexia, loss of appetite and even death in critical stages. 

Serum biochemical parameters of broiler chicken fed diets supplemented with LGO 

Serum biochemical parameters of broiler chickens fed diets supplemented with different levels of LGO is presented in (Table 3). Total protein (TP), albumin, globulin, creatinine and glucose values ranged between 2.64 -3.98 g/dL, 1.09-1.98 g/dL, 1.55-2.00 g/dL, 0.12-0.41 Mg/dl and 105.9-200.8 Mg/dl respectively. The above values were significantly (P<0.05) affected by the dietary supplementation of LGO, values recorded were highest in T4 and T5, in-between in T2 and T3 but lowest in T1. Cholesterol (110.4-191.7 Mg/dl), triglycerides (100.2-151.8 Mg/dl), HDL (108.3-140.9 Mg/dl), LDL (0.52-1.88 Mg/dl), uric acid (3.97-9.52 Mg/dl), ALT (4.07-7.16 u/L), AST (99.90-188.0 u/L) and ALP (40.40-89.50 u/L) respectively. The chlolesterol, LDL, HDL, triglycerides, ALT, AST and ALP values were elevated in T1, mid-way in T2 and T3 and lowest in T4 and T5 (P<0.05). 








Total protein (g/dL)







Albumin (g/dL)







Globulin (g/dL)







Creatinine (Mg/dl)







Glucose (Mg/dl)







Cholesterol (Mg/dl)

Triglycerides (Mg/dl)

HDL (Mg/dl)

LDL (Mg/dl)

























Uric acid (Mg/dl)







ALT (u/L)







AST (u/L)

ALP (u/L)













Table 3: Serum analysis of broiler chicks fed different levels of ATSM Means in the same row with different superscripts differ significantly (P<0.05)

ALP: alanine phosphatase; AST: alanine serum transaminase; ALT: alanine amino transferase. 

Serum electrolytes of broiler chicken fed diets supplemented with LGO 

Table 4 reveals the serum electrolytes of broiler chicken fed diets supplemented with different levels of LGO. Sodium ion ranged between 130.2-136.8 Mmol/L, chloride (100.5-108.1 Mmol/L), calcium (71.11- 83.41Mmol/L) and potassium (5.00-6.84 Mmol/L). The parameters measured were not significantly affected (P>0.05) by the dietary supplementation of LGO. 








Na (Mmol/L)







Cl (Mmol/L)







Ca (Mmol/L)







K (Mmol/L)







Table 4: Serum electrolytes of broiler chickens fed diet supplemented with LGO.

Na: sodium; Cl: chloride; Ca: calcium; K: potassium 

The dry matter values obtained in this experiment are in agreement with the values reported by [25] who examined the effects of aqueous leaf extract on the performance of broiler chicks but contrary to the reports of when turmeric extract rhizome was supplemented in the diet of broiler chickens. Crude protein levels in this study were within the recommended ranges by [26,27]. Crude fiber and ether extract content reported were slightly higher than those reported but in conformity with the values [28,29] who evaluated the effects of Albizia lebbeck  stem bark aqueous extract as alternative to antibiotic feed additives in broiler chicks diets. Calcium and phosphorus content were in accordance with the findings of [30,31] on the influence of two plant extracts on broilers performance, digestibility and digestive organ size. Energy values were in conformity with the findings obtained who determined the effects of black pepper, turmeric powder and coriander seed combination as feed additives on the growth performance of broiler chickens. 

Blood act as a pathological reflector of the status of exposed animals to toxicants and other conditions [32]. It also plays a vital role in the transportation of nutrients, metabolic waste products and gases around the body [33]. Blood constituents change in relation to the physiological conditions of health and provide useful diagnosis and prognosis of disease in animals [34,35]. According to [36-38] haematological studies are can be used as a useful tool to determine the extent of blood damage. The result obtained in this study revealed that increase in PCV levels especially among birds in T4 and T5 had a direct effect with corresponding increase in RBC and Hb values (P<0.05). This result is in agreement with the findings of [39]. However, all values were within the normal physiological range for healthy birds reported [40-42] who reported a PCV range of 25-34 %, RBC 1.5 – 3.0 (mm3 × 106) and Hb (6.5-13.0 g/dL) respectively. Similarly, MCV, MCH, MCHC, WBC and its differentials also increased with increase dietary supplementation of LGO (P<0.05), this is evident especially among birds in T3, T4 and T5.PCV plays a vital role in oxygen, carbon (IV) oxide and nutrients in the body [43], this implies that animals in T4 and T5 will perform better compared to T1, due to sufficient nutrients and gases in the body. Poor nutrition as one of the cardinals of management could trigger low Hb and RBC levels or concentrations, thus making animal’s susceptibility to diseases [44]. RBC 1.22-2.50 (mm3 × 106) and Hb (7.55-11.89 g/dL) range reported in this study are in agreement with the findings [45,46] also reported the lower range of RBC, MCH and MCHC in the blood could be an indication of anaemia. WBC and its differentials are responsible for fighting infections through the production of antibodies, thus, animals with high WBC count have high degree of resistance to diseases [47]. However, the WBC counts reported in this experiment were in conformity to the findings of [48,49] who carried out a comparative studies on the haematological parameters of different strains of broiler chicks (Ross, Arbo acre, Cobb and Arian). Leucocytes counts in this study was less than the values of neutrophils, this is an indication that the anti-nutrient content of LGO is below the lethal levels which agreed with the earlier report by [50,51]. 

According to [52], serum protein may be used as an indirect measurement of dietary protein quality. Total protein is the summation of albumin and globulin concentrations, serum albumin are influenced by age, breed, environment, physiological state and antigen exposure [53]. Albumin and globulin values reported in this study were significantly (P<0.05) different among the dietary treatments. Birds in T3, T4 and T5 had the highest blood protein concentrations; it is also an indication that the protein levels in the diet were enough to support normal protein reserves across the treatments for growth and maintenance [54,55]. According to [56], globulin is an index to measure the immune system and antibody production in the blood. Lower total protein concentration in the blood could be a sign of hypo albuminemia and could advance to liver dysfunction during critical conditions [57,58]. The total protein, albumin and globulin contents observed in this study were within the normal ranges for birds reported [59]. The result obtained is in agreement with the findings of [60-62]. Birds in T4 and T5 had the concentration of glucose, low glucose level in the blood could be attributed to stress, anorexia disturbance in digestion and environment or housing [63] Glucose levels determined in this study were within the range of reference values reported for birds in previous studies [64,65].Uric acid and creatinine are end products of nitrogen and muscle metabolism Nutrition and age are factors that influence the concentrations of urea in birds [66]. High creatinine is indicative of poor protein and amino acid metabolism that can lead to impaired renal function and cardiac infarction The creatinine and urea value obtained in this study were within 0.6-1.2 mg/dl and 5-20 mg/dl reported [67,68] for healthy birds. Cholesterol, triglycerides, HDL and LDL follow similar trends, the values reduced as the level of LGO increased at a significant level (P<0.05). This could be due to the presence of phytochemicals in LGO, capable of modulating the parameter, thus promoting food safety and preventing the incidence of cardiovascular diseases [69-71], high concentrations of cholesterol and LDL in blood serum are the major cause of cardiovascular diseases. The results obtained (LDL, HDL, cholesterol and triglycerides) were in agreement with the reports of [72,73] who examined the haematological and serum biochemical indices of necked neck and normally feathered Nigerian indigenous chickens. Serum enzymes ALT, AST and ALP are estimated to determine liver functions. However, the range of ALT and AST level obtained for birds in this study were similar to 6.94 -8.63 u/L and 147.95 – 192.56 u/L reported [74]. Serum enzyme values slightly decreases as the level of LGO increased at a significant level (P<0.05). This is a clear indication that LGO is non-toxic and the birds have the ability to tolerate the anti-nutrients in the diets; significant elevation of serum enzymes above normal is an indication of pathological disorders in the liver of animals. Result obtained agrees with the findings of [75] but contrary to the reports of who evaluated the blood profile of broiler chickens fed three local sorghum varieties grown in Bauchi State. 

Serum electrolytes follow similar trend, the values were not significantly (P>0.05) influenced by dietary supplementation of LGO. The result obtained is in conformity with the values obtained by [76,77] on the effect of dietary supplementation of hemp (Cannabis sativa) and dill seed on performance, serum biochemical’s of broiler chicken but contrary to the reports of the normal values of serum electrolytes is an indication that the integrity of the liver and kidney were not compromised, thus ensuring health stability in the birds.


Antibiotic resistant strain of bacteria is an increasing threat to animal and human health, these necessitated the use of medicinal plants and their Extract (EOs) which are found to be effective and safe because of their abundant composition of phytochemicals and other nutrients. LGO is found to perform multiple biological activities and its supplementation in the diet of broiler chicks at 0.4 % does not have any deleterious effect on the performance and health status of the animal.


  1. Adu OA, Akingboye KA, Akinfemi G (2009) Potency of pawpaw latex as an anthelmintic in poultry production. Bot. res. Int 2: 139-142.
  2. Galani AH, Rahman AU (2005) Trends in ethnopharmacology. J. Ethnopharmacology 100: 43-49.
  3. Alagbe JO, Ajagbe AD, Attama J, Philemon KC, Kamoru B (2020)Albizia lebbeck stem bark aqueous extract as alternative to antibiotic feed additives in broiler chicks diets: Haematology, Serum indices and oxidative status. International Journal of Biological, Physical and Chemical Studies 2: 8-15.
  4. Oluwafemi RA, Omokore EA, Alagbe JO (2020) Effects of dried water melon and sweet orange peel (DWMOP) meal mixture on the haematological and serum indices of growing rabbits. International Journal of Integrated Education. 3: 244-250.
  5. Burt S (2004) Essential oils: their antibacterial and potential applications in foods a review. Int. J. Food Microbiology 94: 223-253.
  6. Negrelle R, Gomes EC (2007) Cymbopogon citratus: chemical composition and biological activities. RevistaBrasilera de PlantasMedicinaisBotucatu (Brasil) 9: 80-92.
  7. Selim SA (2011) Chemical composition, antioxidant and antimicrobial activity of the essential oil and methanol extract of the Egyptian lemon grass. Grasas aceites 62: 55-61.
  8. Tovar L, Pinto G, Maciel W, Batistella C, Maciel-Filho R, et al. (2011) Short path distillation process of lemon grass essential oil: physiochemical characterization and assessment quality of the distillate and the residue of products. Industrial and Engineering Chemistry Research 50: 8185-8194
  9. Bakkali F, Averbeck S, Averbeck D, Idaomar M (2008) Biological effects of essential oil: A review. Food and Chemical Toxicology 46: 446-475.
  10. Alagbe JO (2020) Caecal Microbial Population of Growing Grass Cutters (ThyronoymsSwinderianus) Fed PhyllantusAmarus and PilogstigmaThonngii Leaf Meal Mixture as Partial Replacement for Soya Bean Meal. Concept of Dairy and Veterinary Sciences 3: 350-355.
  11. Vázquez-Briones MDC, Hernández LR, Guerrero-Beltrán JA (2015) Physicochemical and Antioxidant Properties of Cymbopogon citratesEssential Oil. Journal of Food Research 4: 36-45.
  12. Oluwole SO, Funmilayo EA, David TA, Kehinde AO (2019) Phytochemistry and pharmacological activities of Cymbopogon citratus: A review Scientific African 6: 1-11.
  13. Shah G, Shri R, Panchal V, Sharma N, Singh B, et al (2011) Scientific basis for the therapeutic use of Cymbopogon citratus. Journal Adv. Pharma. Techn 2: 3-8.
  14. Manvitha K, Bidya B (2014) Review on pharmacological activity of Cymbopogon citratus). International Journal of Herbal Medicine 1: 5-7.
  15. Costa G, Grangeria H, Figueirinha IV, Figueiredo M, Batista T (2016) Influence of harvest date and material quality on polyphenol content and antioxidant activity of Cymbopogon citratus infusion. Indust. Crops. Prod 83: 738-745.
  16. Caspar W (2002) Recent advances in Animal Nutrition. A paper presented at an International Symposium held in New Delhi, India.
  17. Hazzit M, Baaliouamer A, Faleiro ML, Miguel MG (2006) Composition of the essential oil of Thymus and Origanum oil spp from Algeria and their antioxidant and antimicrobial activities. J. Agric. Food. Chem 54: 6314-6321.
  18. Karadas F, Pirgozliev V, Rose SP, Dimitrov D, Bravo D, et al. (2014) Dietary essential oils improve the hepatic antioxidant status of broiler chicken. British Poultry Sci 55: 329-334.
  19. Alagbe JO, Grace FR (2019) Effect of Albizia lebbeck seed oil dietary supplementation on the haematological and serum biochemical parameters of weaner rabbits. Sumerianz Journal of Agriculture and Veterinary 2: 96 -100.
  20. Gary DB, Richard DM (2002) Interrelationship between nutrition and immunity. UF/IFAS extension, Gainesville.
  21. Alagbe JO (2019) Haematology, serum biochemistry, relative organ weight and bacteria count of broiler chicken given different levels ofLuffa aegyptiaca leaf extracts. International Journal of Advanced Biological and Biomedical Research 7: 382-392.
  22. Olafadehan OA, Oluwafemi RA, Alagbe JO (2020) Performance, haemato-biochemical parameters of broiler chicks administered Rolfe (Danielliaoliveri) leaf extract as an antibiotic alternative. Advances in Research and Reviews 1: 4.
  23. George W, Latimer (2000) Association of Official Analytical Chemists. Official Methods of Analysis (19th Edition) Washington, D.C.
  24. Duncan DB (1955) Multiple range and multiple F-test. Biometrics 11: 1-42.
  25. Olafadehan OA, Oluwafemi RA, Alagbe JO (2020) Carcass quality, nutrient retention and caeca microbial population of broiler chicks administered Rolfe (Danielliaoliveri) leaf extract as an antibiotic alternative. Journal of Drug Discovery 14: 146-154.
  26. National Research Council (1994) Nutrient requirement of poultry (9th Rev Edn), National Academy Press, Washington D.C.
  27. Barreto MSR, Menten JFM, Racanicci AM, Rizzo PV, Pereira PWZ (2008) Plant Extracts used as Growth Promoters in Broilers. Brazilian Journal of Poultry Science 10: 109-115.
  28. Botsoglou NA, Florou-Panari P, Christaki E, Fletouris DJ, Spais AB, et al. (2002) Effect of dietary oregano essential oil on performance of chickens and on iron-induced lipid oxidation of breast, thigh and abdominal fat tissues. British Poultry Science 43: 223-230.
  29. Alagbe JO, Agubosi OCP, Ajagbe AD, Shittu MD, Balogun OM (2020) Performance, haematology and serum biochemical parameters of growing grass cutters fed Phyllantusamarus and Piliostigmathonningii leaf meal mixture as partial replacement for Soya bean meal. United International Journal for Research and Technology 2: 14-23.
  30. Cross DE, Svoboda K, Mcdevitt RM, Acamovic T (2003) The performance of chickens feed diets with and without thyme oil and enzymes. British Poultry Science 44: 18-19.
  31. Hernandez F, MadridJ, Garcia V, Orengo J, Megias MD (2004) Influence of two plant extracts on broilers performance, digestibility and digestive organ size. Poultry Science 83: 169-174.
  32. Bashir M, Alagbe JO, Betty AM, Omokore EA (2020) Growth performance, caeca microbial population and immune response of broiler chicks fed aqueous extract of Balanites aegyptiaca and Alchornea cordifolia stem bark mixture. United Journal for Research and Technology 2: 13-21.
  33. Doyle D (2006) William Hewson (1739-1774) the father of haematology. British Journal of haematology 133: 375-381.
  34. Aderemi FA (2004) Effects of replacement of wheat bran with cassava root sieviate supplemented or unsupplemented with enzyme on the haematology and serum biochemistry of pullet chicks. Tropical Journal of Animal Science 7: 147-153.
  35. Addass PA, David DL, Edward A, Zira KE, Midau A (2012) Effect of age, sex and management system on some haematological parameters of intensively and semi-intensively kept chicken in Mubi, Adamawa State, Nigeria. Iranian Journal of Applied Animal Science 2: 277-282.
  36. Togun VA, Oseni BSA, Ogundipe JA, Arewa TR, Mustapha F (2007) Effects of chronic lead administration on the haematological parameters of rabbits. A preliminary study. Proceedings of the 41st Conferences of the Agricultural Society of Nigeria 341.
  37. Omokore EO, Alagbe JO (2019) Efficacy of dried Phyllantusamarus leaf meal as an herbal feed additive on the growth performance, haematology and serum biochemistry of growing rabbits. International Journal of Academic Research and Development 4: 97-104.
  38. Shittu MD, Adejumo DO, Ewuola EO, Alaba O, Alagbe JO, et al. (2020) Gut morphometric characteristic and ecological response of broiler starter fed varied levels of protein. Asian Journal of Animal Science 14: 33-39.
  39. Lee KW, Everts H, Kappert HJ, Frehner M, Losa R, et al. (2003) Effects of dietary essential oil components on growth performance, digestive enzymes and lipid metabolism in female broiler chickens. British Poultry Science 44: 450-457.
  40. Subhadarsini M, Silpa MG (2020) Comparative haematology and biochemical parameters of Indigenous broiler chicken. International Journal of Scientific Technology Research 9: 972-978.
  41. Alagbe JO, Motunrade A (2019) Haematological and serum biochemical indices of starter broiler chicks fed aqueous extract of Balanites aegyptiaca and Alchornea cordifolia bark mixture. International Journal of Biological, Physical and Chemical Studies 1: 8-15.
  42. Mahmud M, Maidala A, Dantata IJ, Turaki H (2016) Blood Profile and Serum Biochemical Parameters of Broiler Chickens Fed Three Local Sorghum Varieties Grown in Bauchi State 2: 35-40.
  43. Isaac LJ, Abah G, Akpan B, Ekaette IU (2013) Haematological properties of different breeds and sexes of rabbits. Proceedings of the 18th Annual Conference of Animal Science Association of Nigeria. Abuja, Nigeria 24-27.
  44. Hameed AAA, Salih AM, Fadel Elseed AM, Amasab EO (2013) Effect of feeding untreated or urea treated groundnut hull supplemented with different protein sources on blood parameters of Sudan desert lambs. Online J Anim Feed Res 3: 40-46.
  45. Aldi-Hachesoo B, Talebi A, Asri-Rezaei S (2012) Comparative study on blood profiles of indigenous and Ross-308 broiler breeders. Global Veterinary Journal 7: 238-241.
  46. Sidell BD, Brien KM (2006) when bad things happen to good fish: the loss of haemoglobin and myoglobin expression in Antarctic ice fishes. Journal of Experimental Biology 209: 1791-1802.
  47. Alagbe JO (2017) effect of Miadasan as a dietary supplement on performance, carcass characteristic and blood profile of broiler chicken. Scholarly Journal of Agricultural Science 7: 27-33.
  48. Islam MS, Lucky NS, Literak I, Ahad A, Rahman MM, et al. (2004) Changes of haematological parameters of fayoumi, assil and local chickens reared in Sylhet region in Bangladesh. International Journal of Poultry Science 3: 144-147.
  49. Talebi A, Asri-Rezaei S, Rozeh-Chai R, Sahraei R (2005) Comparative studies on haematological values of broiler strains (Ross, Cobb, Arbo-acres and Arian). International Journal of Poultry Science 4: 573-579.
  50. Omolere JO (2020) Probiotics and medicinal plants in poultry nutrition: A review. United International Journal for Research and Technology 2: 7-13.
  51. Gboshe PN, Ebiloma SO, Shettima I, Boyi PU, Netala J (2020) Haematological traits and serum biochemistry of grass cutters fed Elephant grass supplemented with concentrate. Animal and Veterinary Sciences 8: 29-35.
  52. Kamboh AA, Khan MA, Kaka U, Awad EA, Memon AM, et al. (2018) Effect of dietary supplementation of phytochemicals on immunity and haematology of growing broiler chickens. Ital. J. Anim. Sci 17: 1038-1043.
  53. Simaraks S (2015) The effects of table height on the thickness of neck muscle during computer work. Journal of Science and Technology 27: 425-430.
  54. Bell DJ (1991) Non-protein nitrogen and its fractions in plasma and erythrocytes. In: physiology and biochemistry of domestic fowl. Academic Press Inc. London UK 2: 921-931.
  55. Oiabanji RO, Farinu GO, Akinlade JA, Ojebiyi OO, Odunsi AA, et al. (2007) Studies on Haematological and Serum Biochemical Characteristics of Weaner Rabbits Fed Different Levels of Wild Sunflower (Tithonia diversifoliaHemsl A. Gray) Leaf- Blood Meal Mixture. Internation Journal of Agriculture and Apiculture Research 4: 80-89.
  56. Bowes VA, Julian RJ, Stirtzinger T (1989) Comparison of serum biochemical profiles of male broilers with female broilers and White Leghorn chickens. Can J Vet Res 53: 7-11
  57. Altman RB (1979) Avian Clinical Pathology, Radiology, Parasitic and Infectious Diseases, Proceedings of Amer Amin Hosp Assoc South Bend.
  58. Tumbleson ME, Hutchernson DP, Van Burgeo JT (1976) Serum Protein Concentration and Enzyme activity as a Function of Dye Sex in Miniature Swine Growth. Journal of Animal Science 40: 53-68.
  59. Basit MA, Kadir AA, Loh TC, Aziz SA, Salleh A, et al. (2020) Effects of Inclusion of Different Doses of Persicaria odorata Leaf Meal (POLM) in Broiler Chicken Feed on Biochemical and Haematological Blood Indicators and Liver Histomorphological Changes. Animals 10: 1209.
  60. OlatunjiAK, Alagbe JO, Hammed MA (2015) Effects of varying levels of Moringa olifera leaf meal on performance and blood profile of weaner rabbits. International Journal of Science and Research 5: 803-806.
  61. Alagbe JO, Soares DM, Eimoga MM (2018) Efficacy of Shea butter (Butryospermumparkii)-Neem (Azadirachta indica) leaf meal mixture on performance and carcass characteristics, immune response and blood parameters in broiler chickens. Greener Journal of Agricultural Sciences 8: 42-51.
  62. Oluwafemi RA, Omokore EA, Alagbe JO (2020) Effects of dried water melon and sweet orange peel (DWMOP) meal mixture on the haematological and serum indices of growing rabbits. International Journal of Integrated Education 3: 244-250.
  63. Özkan C, Kaya A, Akgül Y (2012) Normal values of haematological and some biochemical parameters in serum and urine of new zealand white rabbits. World Rabbit Sci 20: 253-259.
  64. Reis JH, Gebert RR, Barreta M, Baldissera MD, Santos ID, et al. (2018) Effects of phytogenic feed additive based on thymol, carvacrol and cinnamic aldehyde on body weight, blood parameters and environmental bacteria in broilers chickens. Microb Pathog 125: 168-176.
  65. Paraskeuas V, Fegeros K, Palamidi I, Hunger C, Mountzouris KC (2017) Growth performance, nutrient digestibility, antioxidant capacity, blood biochemical biomarkers and cytokines expression in broiler chickens fed different phytogenic levels. Anim Nutr 3: 114-120.
  66. Kaneko J, Harvey J, Bruss M (1997) Clinical biochemistry of domestic animals. (5th edn) Academic press, New York 661- 668.
  67. Rubio MS, Laurentiz AC, Sobrane F, Mello ES, Filardi RS, et al. (2019) Performance and Serum Biochemical Profile of Broiler Chickens Supplemented with Piper Cubeba Ethanolic Extract. Braz J Poult Sci 21: 1-8.
  68. Mashayekhi H, Mazhari M, Esmaeilipour O (2018) Eucalyptus leaf powder, antibiotic and probiotic addition to broiler diets: Effect on growth performance, immune response, blood components and carcass traits. Animal 12: 2049-2055.
  69. Alagbe JO, Sadiq MR, Anaso EU, Grace FR (2019) Efficacy of Albizia lebbeck seed oil on the growth performance and carcass characteristics of weaner rabbits. Sumerianz Journal of Agriculture and Veterinary 2: 116-122.
  70. Mahmoud A, Shaaban SE, Mayada RF, Mohammed EA, Asmaa EK, et al. (2019) Omega-3 and Omega-6 fatty acids in poultry nutrition: Effect on production performance and health. Animals 9: 573-584.
  71. Stanley J (2010) Dietary cholesterol, blood cholesterol and cardiovascular disease. Lipid Technology 22: 110-112.
  72. Pampori Z, Iqbal S (2007) Haematology, serum chemistry and electrocardiographic evaluation in native chicken of Kashmir. International Journal of Poultry Science 6: 578-582.
  73. Ladokun A, Yakubu A, Otite J, Omeje J, Sokunbi O, et al. (2008) Haematological and serum biochemical indices of naked neck and normally feathered Nigerian indigenous chickens in a sub humid tropical environment. International Journal of Poultry Science 7: 55-58.
  74. Alam M, Ullah MO, Malik SUF, Islam MS (2020) Broiler and indigenous chickens: a comparison through biochemical parameters. International Journal of Sustainable Agricultural Research 7: 228-233.
  75. Hernández F, García V, Madrid J, Orengo J, Catalá P (2006) Effect of formic acid on performance, digestibility, intestinal histomorphology and plasma metabolite levels of broiler chickens. Br Poult Sci 47: 50-56.
  76. Odetola OM, Adejinmi OO, Owosibo OA, Banjo OT, peters OO (2019) Growth Response, Serum Biochemistry and Organ Histopathology of Broilers Fed Diets supplemented with Graded levels of Petiveriaalliacea Root Meal. Int J Poult Sci 18: 45-50.
  77. Vispute MM, Sharma D, Mandal AB, Rokade JJ, Tyagi PK (2019) Effect of dietary supplementation of hemp (Cannabis sativa) and dill seed (Anethum graveolens) on performance, serum biochemicals and gut health of broiler chickens. J Anim Physiol Anim. Nutr 103: 525-533.

Citation: Alagbe JO (2020) Effect of Dietary Supplementation of Cymbopogon Citratus Oil On The Haematology And Serum Biochemical Parameters Of Broiler Chicks. J Biotech Res Biochem 3: 011

Copyright: © 2020  Alagbe JO, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

© 2021, Copyrights Herald Scholarly Open Access. All Rights Reserved!