Journal of Toxicology Current Research Category: Environmental science Type: Research Article
Toxocariasis in Two Ecosystems in Argentina
- Graciela Inés Santillán1*, Graciela Cespedes1, Katherina Viscaychipi1, Irma Sommerfeld2
- 1 Instituto Nacional De Enfermedades Infecciosas Carlos G Malbran Anlis, Buenos Aires, Argentina
- 2 Catedra De Salud Publica Facultad De Veterinaria, Universidad De Buenos Aires, Buenos Aires, Argentina
*Corresponding Author:Graciela Inés Santillán
Instituto Nacional De Enfermedades Infecciosas Carlos G Malbran Anlis, Buenos Aires, Argentina
Received Date: Mar 27, 2018 Accepted Date: May 02, 2018 Published Date: May 16, 2018
2. The second selected area was the Department of Eldorado, in the Mesopotamian sub region, located in the northwest region of Misiones Province. This is an area of 1,927km with 78,152 inhabitants. This region has a tropical climate and no dry season. Northeast, Southeast and East are the prevailing winds. This biome consists in Iguazu tropical rainforest and gallery forest.
There are no adequate conditions of sanitation in the two departments in regards to water inside the house, and sewers. In Malargüe, found a 27.02% of dogs infected and 14.2% of samples with ascarids in the soil of house. Eldorado, stool samples showed a 61.9% of eggs of Toxocara canis. Finding a 64.2% of house contaminated with ascarids. In the two departments, 25% of the studied population had antibodies anti Toxocara canis. The presence of eggs in the soil of the two departments, show significant differences with 0.02755 confidence interval/0.57562 with 95% confidence. Finally we can conclude the findings of the present study show the zoonotic potential of this parasite, revealing the need to implement preventive health-care and educational measures, and to reassert the importance of Toxocariasis as a relevant public health problem.
A study conducted in three provinces of Argentine Patagonia revealed a high potential for environmental contamination with Toxocara spp. eggs (35.1%). However, the seroprevalence was 31.6%, suggesting that extreme weather of the Patagonian steppe inhibits the development of Toxocara spp. eggs .
This study was aimed to evaluate and compare the situation of Toxocariasis in two Argentine ecosystems at different altitudes, demographics, climatic and environmental conditions, as well as political, cultural and health systems: The Department of Malargüe in Mendoza Province, and the Department of Eldorado in Misiones Province.
MATERIALS AND METHODS
The second selected area was the Department of Eldorado, in the Mesopotamian sub region, located in the northwest region of Misiones Province. This is an area of 1,927 km with 78,152 inhabitants [13,14]. This region has a tropical climate and no dry season. Northeast, southeast and east are the prevailing winds. This biome consists in Iguazu tropical rainforest and gallery forest .
Secondary sources were investigated, such as the Argentina Population and Housing Census 2001, 2010, 2011 [13,16,17] and literature on climate and geographical situation of the two study regions [11,15,18]. Also, data on the health situation in the region of Cuyo and Northeastern Argentina (NEA) provided by the Ministry of Health of Argentina [19-22] and reports from the Secretariat of Agriculture, Livestock, Fisheries and Food  of the Center for the Implementation of Public Policies Promoting Equity and Growth (CIPPEC)  and the Directorate of Statistix and Economic Research from Cuyo (DEIE) [22,25] were also analyzed in order to know the regional aspects related to the demographics and socioeconomic dimensions as well as the organization of health services.
The health indicators selected were: Demographic indicators, include population pyramids and dependency ratios; environmental indicators, involve percentage of population with access to drinking water and sanitation, water resources and probability of natural hazards (flood, geological, geomorphological); socioeconomic and political indicators such as gross national product per capita, literacy rate, predominant economic activity, health expenditure, health system and population health insurance coverage.
Blood samples were obtained by venipuncture after the subject or parents of the minors signed an informed consent. A structured form was completed containing basic demographic data such as age, gender, job position, ownership, animals, deworming of cats/dogs, type of anthelmintics used, diet, water supply and type of drainage and wastewater disposal. Serum samples were stored at -20°C until use. Research on human subjects was carried out in accordance with the provisions of the Universal Declaration of Human Rights of 1948, the ethical standards established by the Nuremberg Code in 1947, the Declaration of Helsinki of 1964 and subsequent amendments already regulated by the National Law 25.326 on data protection of individuals [26,27].
Collection of faecal samples from dogs
Collection of soil samples
Telemann method modified : It was performed according to the procedure developed by Telemann, 1908. Samples were observed microscopically using 40x and 10x lenses.
Willis flotation method : It was performed according to the technique developed by Willis, 1921. Samples were observed under a microscope using 40x and 10x lenses.
Sheather method : It was performed according to the procedure developed by Sheather, 1923. Samples were observed under a microscope using 40x lenses.
Total antigen ELISA assay: It was performed according to the technique described by Cerverizzo I et al. , with changes made in the Department of Parasitology of the INEI “Carlos G. Malbrán” ANLIS. In flat bottom inmunolon II 50µl of the antigen ES/L3 total, was coated, settled for 18 hours in the refrigerator. Washed 3 times with buffer (PBS pH 7.2/0.1% Tween 20) P/T for 5 minutes. Was blocked with PBS buffer pH 7.2/skim milk 1.5% for 1 h at 37°C. Incubated with 50µl of dilution of sera of patients with serum controls for 30 min at 37°C in a humid chamber. The wells were once again washed and incubated with 50μl of anti-human IgG (Sigma A 8667) marked with peroxidase, at a concentration of 1/5000, diluted with P/T. Washings were repeated and reaction is revealed by adding 100μl substrate acid 2, 2-Azino-bis (3-etilbenzotiazolin-6) sulfonic acid (ABTS), were incubated plates 10 min, the reaction stopped with 100μl of 0.1-N, pH 3.2 hydrofluoric acid, and was read at 410nm on a team Dynatech MR 4100.
In the Department of Malargüe, the Potential Dependency Ratio (PDR) is almost 55% similar to the whole country, but it is interesting to note that the PDR of the Department of Eldorado is 60% .
The environmental indicators are shown in table 1 [11,13,15].
|Population with access to drinking water||82%||77%|
|Population with access to sanitation||1.76%||4.3%|
|Water resources||Melt-water rivers||Large numbers of rivers|
|Probability of natural hazards (floods, geological and geomorphological hazards)||Volcanic eruptions||Floods|
Table 1: Environmental indicators: Departments of Malargüe and Eldorado.
Political indicators, only the population health insurance coverage was available but data on the number of physicians per 1000 inhabitants in the Department of Eldorado was not available to compare the two departments (Table 2).
|Gross domestic product per capita||1,687 million pesos||4,617 million pesos|
|Prevailing economic activity||Timber industry||Oil industry|
|Population health insurance coverage||38%||39%|
Table 2: Socioeconomic, political and health indicators: Departments of Eldorado and Malargüe.
|Department||Malargüe||Families||Person (n)||Western Blot (+)||%|
|Department||Samples with Ascarids Eggs in Soil||Samples with Toxocara Eggs in Faeces||% of Dogs with Toxocara Eggs||% of Ascarids Eggs in Soil|
|Dogs Found to be Toxocara|
|Negative||10 %||75 %||7.5%||65%|
Table 5: Relationship between the percentage of positive serology and dogs found to be Toxocara carriers in the Departments of Malargüe and Eldorado.
Toxocariasis is little known by pet owners who lack ideas on ways to take necessary measures to minimize infection risks. The main factor for contracting this parasitosis is the presence of animals parasitized with Toxocara spp. in the environment, a situation that causes soil contamination with parasite eggs [34,35]. Several studies conducted worldwide have shown increased rates of soil contamination in urban settings such as parks, public gardens, recreation areas, squares and sandpits [33,34]. However, studies in the United States and Southwestern France showed that people living in rural regions had a higher risk of becoming infected with Toxocara eggs than those living in urban areas [4,6].
In this work two different ecosystems such as the Department of Malargüe (Mendoza) and Eldorado (Misiones) with uneven sociocultural and environmental conditions were evaluated. A significant population growth was observed in both departments which was demonstrated by the high Potential Dependency Ratio (PDR)  leading to an increase in the canine population, 25,000 dogs in Malargüe, and therefore increasing the risk of contracting this parasitosis.
The sanitation level is another parameter of additional exposure that may interact with the infectious power of contaminated soils as stated elsewhere [2,3,10,34,35]. In this study the health system is inadequate in both departments, although the Department of Malargüe is in better conditions since 78.9% of the population uses motor pumps.
It could also be observed that both departments have seriously a very poor sewer connection. In the Department of Malargüe, only 1.76% of the population has a sewer network and 4.3% in the Department of Eldorado. It is very striking that more than half of the population in Malargüe, regardless of gender, has no health coverage at all [18,22].
It can be observed that the NEA is the Argentine region that has allocated less money to the health sector regarding its public spending, representing 11.1% of the 10,843 million pesos spent in 2006 . Of note, in the Department of Malargüe, besides being pets, dogs perform rural activities and therefore defecate elsewhere, spreading parasites in a wider territory that can be favored by western winds blowing at a constant speed, which is related to the results found in this study, since despite we found 27.02% of infected dogs, only 14.2% of ascarid samples were found in houses.
Most of the houses studied in Eldorado had more than one dog and the faecal samples represented 61.9% of Toxocara canis eggs. Note worthy, these houses had no fences, and as a result, dogs wandered along public spaces contaminating the entire surrounding area with Toxocara canis eggs accounting for 64.2% of house contaminated with ascarids, even the floors of the houses with no dogs.
Likewise, the habit of walking barefoot in Eldorado and playing on the floor, besides the poor management of excreta, makes it the optimal scenario for the transmission of Toxocariasis. However, despite the differences in climate, social factors related to health care, state of housing, sanitary conditions and habits and behavior of the population, the serological result from both populations measured by WB have a similar outcome (Table 3). There might be an ecosystem in balance; diseases do not represent a danger but a factor of “natural selection”. Human activities and alterations of the environment have created new dynamics and new patterns for infectious diseases that favour the spread of pathogens, both geographical and between species .
Regarding the percentage of soil and faecal samples positive for Toxocara spp. (Table 4), a greater rate can be observed in the Department of Eldorado. This could have been influenced by the time of year when the sampling was conducted besides the weather condition, favorable for the parasite that needs moisture and room temperature, a suitable substrate for the larvae to develop, considering that Toxocara spp. eggs need to spend time on the floor to be able to infect humans. Misiones Province, as described before, presents a bioenvironmental diversity favorable for maturation and retention of Toxocariasis in humans and animals. The results found in this study are in line with those found in other subtropical areas [3,37,38].
The value of infestation found in this study is close to that reported for the city of Santa Fe (25%), located in Santa Fe Province . Regarding the relationship between positive serology and its association with keeping dogs in the two regions studied (Table 5) there are no significant differences in their percentages as to conclude that animal keeping is a determining factor for the human population to acquire this parasitosis. (The two-sample proportions test, using statistix 8.0, showed no significant difference in CI 95%; limits were -0.32225/0.38151).
This study showed that the presence of Toxocara spp. was more frequent on the floor of houses in the Department of Eldorado than in Malargüe (64.2% vs. 14.2%). The two-sample proportions test using statistix 8.0 revealed that the presence of eggs in the soil of the two Departments present significant differences, CI 95% (0.02755 / 0.57562).
This demonstrates the importance of the common environment and the interaction with contaminated areas and the persistence of parasite eggs as an impact factor to consider in future decision-making activities involving the health-care system.
We can conclude that the geographic distribution of Toxocara spp. depends largely on weather conditions, soil types and the behavior of susceptible populations. The findings of the present study show the zoonotic potential of this parasite, revealing the need to implement preventive health-care and educational measures, and to reassert the importance of Toxocariasis as a relevant public health problem.
- Alonso JM, Luna C, Fernandez G, Bojanich MV, Alonso ME (2006) Toxocara eggs in recreational soils in an Argentine city. Acta Bioquím Clín Latinoam 40.
- Fonrouge R, Guardis MV, Radman NE, Archelli SM (2000) Soil contamination with Toxocara sp. eggs in squares and public places from the city of La Plata. Buenos Aires, Argentina. Bol Chil Parasitol 55: 83-85.
- Holland C, Smith HV (2006) Toxocara: The enigmatic parasite. CABI International, Wallingford, UK.
- Martín UO, Demonte MA (2008) Urban contamination with zoonotic parasites in the central region of Argentina. Medicina (B Aires) 68: 363-366.
- Mizgajska H, Luty T (1998) [Toxocariasis in dogs and contamination of soil with Toxocara spp. eggs in the Pozna? region]. Przegl Epidemiol 52: 441-446.
- Sommerfelt I, Degregorio O, Barrera M, Gallo G (1992) Presencia de huevos de Toxocara spp. En paseos públicos de la ciudad de Buenos Aires, Argentina, 1989-90. Rev Med Vet 73: 70-74.
- Sommerfelt IE, Cardillo N, López C, Ribicich M, Gallo C, et al. (2006) Prevalence of Toxocara cati and other parasites in cats' faeces collected from the open spaces of public institutions: Buenos Aires, Argentina. Vet Parasitol 140: 296-301.
- Liberato C, Berrilli F, Odorizi L, Scarcella R, Barni M, et al. (2018) Parasites in stray dogs from Italy: Prevalence, risk factors and management concerns. Acta Parasitol 63: 27-32.
- Fillaux J, Santillan G, Magnaval JF, Jensen O, Larrieu E, et al. (2007) Epidemiology of toxocariasis in a steppe environment: The Patagonia study. Am J Trop Med Hyg 76: 1144-1147.
- Fontanarrosa M, Vezzani D, Basabe J, Eiras D (2006) An epidemiological study of gastrointestinal parasites of dogs from Southern Greater Buenos Aires (Argentina): Age, gender, breed, mixed infections, and seasonal and spatial patterns. Vet Parasitol 136: 283-295.
- INDEC (2010) Censo Nacional de Población, Hogares y Viviendas. INDEC, Buenos Aires, Argentina.
- INDEC (2001) Censo Nacional de Población, Hogares y Viviendas. INDEC, Buenos Aires, Argentina.
- INDEC (2011) Censo Nacional de Población, Hogares y Viviendas. INDEC, Buenos Aires, Argentina.
- Ministerio de Salud (2008) Análisis de la situación de salud región Cuyo Noviembre.
- Ministerio de Salud (2008) Análisis de la situación de salud región NEA Noviembre.
- INDEC (2006) SAGPyA (Secretaria de agricultura, ganadería, pesca y alimentación).
- DEIE, Área Indicadores de Coyuntura - y Facultad de Ciencias Económicas, UNCuyo.
- Oshima S (1966) Review of studies on visual acuity and function in 1965. Ganka 8: 455-472.
- Telemann W (1908) Eine methode zur erleichterung der auffindung von parasiteneiern in den faeces. Dtsch med Wochenschr 35: 1510-1511.
- Willis H (1921) A simple levitation method for the detection of hookworm ova. Med J Australia 29: 375-376.
- Sheather AL (1923) The detection of intestinal protozoa and mange parasites by a flotation technique. Journal of Comparative Pathology and Therapeutics 36: 266-275.
- Santillán G, Bastin V, Céspedes G, Monkiewicz A (2013) [Evaluation of a purified antigen for the diagnosis of toxocariosis]. Rev Argent Microbiol 45: 80-85.
- Cerverizzo I, Santillán G, Cabrera M, Bellegarde E (1990) Inmunodiagnostico en Toxocariasis. Boletín de la Asociación Argentina de Microbiología 85: 14-15.
- B?aszkowska J, Góralska K, Wójcik A, Kurnatowski P, Szwabe K (2015) Presence of Toxocara spp. eggs in children's recreation areas with varying degrees of access for animals. Ann Agric Environ Med 22: 23-27.
- Bojanich MV, Alonso JM, Caraballo NA, Itatí Schöller M, López Mde L, et al. (2015) Assessment of the presence of Toxocara eggs in soils of an arid area in central-western Argentina. Rev Inst Med Trop Sao Paulo 57: 73-76.
- Cocianic P, Zonta ML, Navone GT (2018) A cross-sectional study of intestinal parasitoses in dogs and children of the periurban area of La Plata (Buenos Aires, Argentina): Zoonotic importance and implications in public health. Zoonoses Public Health 65: 44-53.
- Magnaval JF, Glickman LT, Dorchies P, Morassin B (2001) Highlights of human toxocariasis. Korean J Parasitol 39: 1-11.
- Mizgajska H (2001) The role of environmental factors in the contamination of soil with Toxocara spp. and other geohelminth eggs. Parasitol Int 46: 67-72.
Citation: Santillán GI, Cespedes G, Viscaychipi K, Sommerfeld I (2018) Toxocariasis in Two Ecosystems in Argentina. J Toxicol Cur Res 2: 003.
Copyright: © 2018 Graciela Inés Santillán, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.