Journal of Aquaculture & Fisheries Category: Aquaculture Type: Original Article

Effects of Dietary Supplementation of Zingiber officinale Root-Powder on Growth, Nutrient Utilization and Intestinal Microbes of African Mud Catfish (Clarias gariepinus) Fingerlings

Sherifat Ibidunni Adegbesan1*, Samuel Olubodun Obasa1, Aderonke Kofoworola Akintokun2 and Ikililu Abdulraheem1
1 Department of aquaculture and fisheries management, College of Environmental Resources Management, Federal University of Agriculture, Abeokuta, Nigeria
2 Department of microbiology, College of Biological Sciences, Federal University of Agriculture, Abeokuta, Nigeria

*Corresponding Author(s):
Sherifat Ibidunni Adegbesan
Department Of Aquaculture And Fisheries Management, College Of Environmental Resources Management, Federal University Of Agriculture, Abeokuta, Nigeria
Tel:+234 8167846123,
Email:ibidunnisherifat@yahoo.co.uk

Received Date: Mar 04, 2019
Accepted Date: Mar 12, 2019
Published Date: Mar 26, 2019

Abstract

Phytobiotics are valuable materials in promoting growth and reducing pathogenic diseases in cultured fish diets. To this effect, a 12 weeks experiment was conducted in a 40 litres fresh water filled rectangular plastic tanks to evaluating the growth, nutrient utilization potential and gasytointestinal microbes of Clarias gariepinus fed varying levels of Zingiber officinale root powder. 120 C. gariepinus fingerlings (2.33 ± 0.07 g average weight) were fed with 40 % crude protein diets containing three concentrations of Z. officinale roots-powder: ZOP1-1 %; ZOP2-2 %; ZOP3-3 %, and control-0 % ad libitum twice daily for 12 weeks. Significant differences (p < 0.05) occurred in the growth and nutrient utilization parameters except feed conversion ratio and specific growth rate.

Survival rate decreased as concentration of powder increased. The Total Bacterial Counts (TBC) and Total Fungal Counts (TFC) decreased as inclusion levels of the supplements increased which were different (p < 0.05) from the control. The study concluded that 1 % Z. officinale roots-powder dietary supplementation in cultured C. gariepinus could effectively promote growth and reducing the microbial load of the fish.

Keywords

Clarias gariepinus; Dietary supplement; Growth; Microbe; Z. officinale roots-powder

INTRODUCTION

Aquaculture, one of the most important options in animal protein production. It however requires high quality feeds with high protein content and some complementary additives or dietary supplements to keep fish healthy and to enhanced growth [1]. Medicinal plant products (phytobiotics) have been shown to act as anti-stress, growth promoters, appetite stimulators, tonic and immunostimulants and to have antimicrobial characteristics in finfish and shell fish culture [2]. Phytobiotics are plant-derived, natural compounds embedded into diets which enhanced animal productivity and they include: Aloe vera (Aloe barbadensis), garlic (Allium sativum) and ginger (Zingiber officinale) as growth promoters have been widely used in different species of fish [3]. All fish live in microbe-rich environment and are vulnerable to invasion by pathogenic and opportunistic micro-organisms. The environment of fish being aquatic is very challenging with fish in constant interaction with a wide range of pathogenic and non-pathogenic micro-organisms [4].

To develop alternative practices for growth promotion and disease management in aquaculture, attention has also been focused in identifying novel drugs, especially from plant sources. Rao et al. [5], reported that these drugs may be delivered to the cultivable organisms either through feed supplementation or oral delivery through diets mode.

(Z. officinale) belongs to the Zingiberaceae plant [6]. It is beneficial to growth and immune systems in aquatic animals [7]. The rhizome of Z. officinale has been reported to possess a broadspectrum of prophylactic and therapeutic activities [8]. It is important in fish diet in that it control infection [9]. The function the plant performed is linked to the active ingredients that are present in the plant. Themajor constituents in ginger rhizomes are carbohydrates (50-70 %), lipids (3-8 %), terpenes, and phenolic compounds [10]. Hence, this study aims at assessing the growth, nutrient utilization and anti-microbial potentials of cultured Clarias gariepinus [11] fed varying inclusion levels of Z. officinale root-powder.

Experimental system

The study was conducted at the fish farm (hatchery unit) of the Department of Aquaculture and Fisheries Management, College of Environmental Resources Management, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria. The experiment was conducted in twelve (12) rectangular plastic tanks each (60 litres) and each tank was two third filled (40 litres).

Experimental fish

African mud catfish (C. gariepinus) fingerlings (2.33 g average weight) were used in this study. A total of one hundred and twenty (120) fingerlings were purchased at Motherhood Fish Farm, Abeokuta, Ogun State, Nigeria. The fish were randomly (completely randomized design) allotted into four (4) treatments in the plastic tanks at a stocking rate of ten fingerlings per tank in triplicates.

Experimental diets

Four iso-nitrogenous diets containing 40 % Crude Protein (CP) were formulated to containing three diets containing different concentrations of Z. officinale root-powder and the control as listed below:

• Treatment 1 (Control) - 0 % 
• Treatment 2 (ZOP1) - 1 % Z. officinale root-powder
• Treatment 3 (ZOP2) - 2 % Z. officinale root-powder
• Treatment 4 (ZOP3) - 3 % Z. officinale root-powder

Diets formulation and preparation

A ration of 40 % Crude Protein (CP) containing fishmeal (72 % CP), groundnut cake (45 % CP), soybean meal (42 % CP) and yellow maize (10 % CP) as the energy source and fixed ingredients including vitamin premix (1 %), lysine (0.5 %), methionine (0.5 %), di calcium phosphate (0.5 %); salt (0.5 %) and vegetable oil (4.0 %). The weight of each ingredient was calculated using Pearson Square method using the stipulated crude protein requirement of the fish. All the four diets formulated were carefully prepared which involves measuring the ingredients, thoroughly mixing the ingredients, and pelletizing them as described by Fagbenro and Adebayo [12]. The ginger rhizome was prepared separately before incorporation into the basal diets.

Preparation and processing of Z. officinale root-powder

Fresh rhizomes of Z. officinale roots were bought from a local market in Abeokuta, Ogun state, Nigeria and were confirmed by a botanist. The plant was dried in the shade. The dried rhizomes were further grinded into powdered form using a household grinder and sieved using a household sieve as described by Haghighi and Rohani [13].

Incorporation of Z. officinale root-powder into the diets

The powdered Z. officinale root produced was mixed directly with the basal diet and added into the diets at concentration of 1 %, 2 % and 3 % of feed. Compounded feeds were pelletized (2 mm) using the pelletizing machine from University fish farm, sun dried and allowed to cool then packed and stored in an opaque nylon bag according to the treatments. The percentages of all the feed ingredients used are shown in table 1.

Ingredients (%)

Control

ZOP1

ZOP2

ZOP3

Fishmeal

31.2

31.2

31.2

31.2

Soybean meal

15.6

15.6

15.6

15.6

Groundnut cake

15.6

15.6

15.6

15.6

Yellow Maize

30.5

30.5

28.75

27.75

Vitamin Premix

1.0

1.0

1.0

1.0

Lysine

0.5

0.5

0.5

0.5

Salt

0.5

0.5

0.5

0.5

Vegetable oil

4.0

4.0

4.0

4.0

Methionine

0.5

0.5

0.5

0.5

DCP

0.5

0.5

0.5

0.5

Z. officinale root-powder

0.0

1.0

2.0

3.0

Total

100

100

100

100

Table 1: Feed ingredients and Proximate Compositions of the Experimental Diets (% Dry weight) 
DCP= Di Calcium Phosphate

PROXIMATE ANALYSIS

The Proximate analysis of the four diets and the fish were done following procedure as described by AOAC [14]. The proximate composition of Z. officinale root-powder [15] is shown in table 2.

Parameter

Amount (%)

Crude protein

34.13

Crude fat

4.02

Ether extract

4.07

Ash content

7.64

Moisture content

13.75

Vitamin C

1.036

Table 2: Chemical composition of ginger root powder.

Experimental procedure

The fish were acclimated to the experimental system for a period of 14 days before starting the experiment and were fed two times daily with a commercial diet (40 % CP). The fish were weighed according to the set up per treatments at the beginning of the experiment.

Fish feeding

All fish were starved for 24 hours to increase the appetite of the fish before starting the experiment. Fish were fed with the diets two times daily, in the morning between 09:00 - 10:00 h and evening between 16:30 - 17:30 h, ad libitumfor 12 weeks.

Water quality management

Water temperature (°C) and dissolved oxygen (mg \ l) were measured every week using a combined digital YSI dissolved oxygen meter (YSI Model 57, Yellow Spring Ohio); pH was monitored weekly using pH meter (Mettler Toledo-320, Jenway UK).

MONITORING OF FISH GROWTH

Weighing of the fish were done in each tank weekly to monitor the fish growth and ensure adequate feed consumption using a sensitive electronic weighing scale (Mettler Toledo FB602, Jenway UK).

DETERMINATION OF GASTROINTESTINAL MICROBES OF THE FISH

Estimation of bacterial counts

The ventral surface of the fish was dissected to removing the gut proximal section. The fish guts (intestines) were gotten for estimation the precision of serial dilution of viable bacteria count and fungi counts as described by Hedges [16].

Microbial identification

The bacterial culture were isolated and were subjected to morphological and biochemical tests for their identification according to the methods of Buchanan and Gibbson [17]. The results were analyzed by cross reference to Bergey’s Manual of Systematic Bacteriology [17].

The fungal isolates were subjected to morphological characteristics according to Barnett and Hunter [18] and the isolated fungi were identified according to Campbell et al. [19].

DATA ANALYSIS

Analysis of fish growth performance

Growth performance of fish was done as illustrated by Agbebi et al. [20], in term of mean weight gain, percentage weight gain, survival (%), specific growth rate (SGR % / day). The growth parameters calculated at the end of the experiment were:


Analysis of feed conversion and nutrient utilization

The nutrient utilization parameters such as the Feed Conversion Ratio (FCR), Protein Efficiency Ratio (PER) and Net Protein Utilization (NPU) responses were calculated and data were obtained from them.

STATISTICAL ANALYSIS

The data obtained were subjected to one way Analysis of Variance (ANOVA). Means were separated using Duncan Multiple Range Test [21] at a significant level of 0.05. Computations were subjected to SAS statistical package version 15.0.

RESULTS

Proximate composition of experimental diets

Proximate compositions of the four diets formulated and prepared for the feeding trial are presented in table 3. Crude protein of the experimental diets is 40 % among the four treatments, moisture content ranged between 9.56 and 11.0 %, ether extract ranged between 4.70 and 5.42 %, fibre contents ranged between 1.23 and 1.67 %.

Proximate components (%)

Control (0 %)

ZOP1 (1 %)

ZOP2 (2 %)

ZOP3 (3 %)

Moisture

10.50

10.98

9.86

9.56

Crude protein

40.01

40.00

40.04

39.98

Fibre content

3.10

3.12

3.04

3.42

Ash

5.20

4.45

3.95

3.74

Ether extract

5.42

5.20

4.98

4.70

Nitrogen free extract

35.77

36.25

38.13

38.60

Table 3: Proximate compositions of the experimental diets (% Dry weight).

Carcass compositions of experimental fish

The initial and final carcass compositions of the fish fed with varying levels of ginger root-powder and the control is presented in table 4. The crude protein content of the fish carcass ranged between 43 and 52.5 %.

Proximate components (%)

Initial

Control (0 %)

ZOP1 (1 %)

ZOP2 (2 %)

ZOP3 (3 %)

Moisture

11.54

11.84 ± 0.23b

11.92 ± 0.02a

11.85 ± 0.03a

11.97 ± 0.04a

Crude protein

43.50

47.37 ± 0.55c

52.48 ± 0.65a

50.83 ± 0.17b

50.19 ± 0.38b

Fibre content

0.90

1.23 ± 0.02c

1.34 ± 0.05b

1.60 ± 0.12a

1.39 ± 0.04b

Ash

0.98

4.18 ± 0.31a

3.83 ± 0.13b

3.49 ± 0.01b

3.57 ± 0.04b

Ether extract

8.50

12.44 ± 0.08a

9.85 ± 0.20b

10.01 ± 0.28b

9.58 ± 0.06b

Nitrogen free extract

34.58

22.95 ± 0.09a

19.58 ± 0.38c

21.22 ± 0.60b

23.30 ± 0.36a

Table 4: Proximate compositions of the fish (% Dry weight) (Mean ± SEM).
Means along the same row with same letter are not significantly different (p > 0.05).

Physicochemical parameters of the water

The physicochemical parameters of the water recorded during the experimental period are shown in table 5.

Week

pH

Dissolved Oxygen (Mg / L)

Temperature (°C)

0

6.00

6.10

25.00

1

6.90

6.20

25.41

2

7.15

6.18

25.60

3

7.03

6.24

25.78

4

7.34

6.35

25.83

5

7.41

6.50

25.61

6

7.48

7.21

26.13

7

7.31

7.25

25.53

8

7.22

7.40

26.20

9

7.28

7.56

26.13

10

7.50

7.45

26.30

11

7.55

7.61

26.05

12

7.56

7.70

26.02

Table 5: Physicochemical Parameters of water during experimental period.

GROWTH PERFORMANCE

The growth performance and nutrient utilization of C. gariepinus fed Z. officinale root-powder at three varying levels of dietary supplementation and the control is shown in table 6. There were differences in the mean Weight Gains (WG) among fish fed Z. officinale root-powder. There were significant differences in the mean weight gain among fish fed with the Z. officinale root-powder when compared to the fish fed with the control diets. The highest WG (18.0 g) and Percentage With Gain, PWG (838 %) was recorded in fish fed with 1 % Z. officinale root-powder while the lowest WG (11.9 g) and PWG (518 %) was recorded in fish fed with control diet. The highest Feed Conversion Ratio, FCR (1.57) was observed in fish fed with 1 % Z. officinale root-powder while the lowest FCR was recorded in fish fed with control diet. There were significant no differences in the protein efficiency ratio among fish fed with the Z. officinale root-powder.

Parameters

Control (0 %)

ZOP1 (1 %)

ZOP2 (2 %)

ZOP3 (3 %)

Initial weight (g)

2.30 ± 0.06a

2.33 ± 0.09a

2.33 ± 0.03a

2.33 ± 0.09a

Final weight (g)

14.22 ± 1.12c

20.27 ± 0.92a

18.49 ± 1.09b

20.14 ± 1.15a

Weight gain (g)

11.92 ± 1.16c

17.95 ± 0.90a

16.15 ± 1.06b

17.82 ± 1.07a

Percentage weight gain (%)

518.3 ± 10.21c

840.8 ± 7.70a

691.4 ± 10.63cb

762.3 ± 17.69b

Feed intake (g / fish)

19.17 ± 2.29c

27.11 ± 1.49a

25.2 ± 1.65b

25.64 ± 1.96b

Feed conversion ratio

1.40 ± 0.07b

1.57 ± 0.04a

1.56 ± 0.02a

1.55 ± 0.02a

Specific growth rate (% / day)

2.31 ± 0.19b

2.57 ± 0.10a

2.46 ± 0.06a

2.56 ± 0.02a

Protein efficiency ratio

1.80 ± 0.09a

1.65 ± 0.04b

1.60 ± 0.02b

1.61 ± 0.03b

Apparent net protein utilization (%)

50.29 ± 1.38c

83.04 ± 3.92a

68.270 ± 5.12b

65.60 ± 5.05b

Survival rate (%)

86.67 ± 8.82a

80.00 ± 5.77b

63.33 ± 3.33c

63.33 ± 3.33c

Table 6: Growth performance and nutrient utilization of Clarias gariepinus fed Z. officinale root-powder supplemented diets (Mean ± SEM).
Means along the same row with same letter are not significantly different (p > 0.05).

INTESTINAL MICROBES OF THE FISH

Microbial count of fish

The total bacteria count and fungal counts in the intestine of fish fed Z. officinale root-powder diets and the control are presented in table 7. The microbial population of fish fed diet containing varying levels of ginger roots powder was greatly reduced compared to the control (p < 0.05). A significant difference (p < 0.05) occurred in the total bacterial and a fungal count was recorded among the treatment groups with the control having the highest count. Fish fed 2 % Z. officinale root-powder diet recorded the least bacterial and fungal counts followed by 3 % Z. officinale root-powder fed diet.

Parameters

Control 0 %

ZOP1 1 %

ZOP2 2 %

ZOP3 3 %

Total Bacteria Count (CFU / ml) × 105

23.67 ± 0.88a

17.33 ± 4.76b

7.4 ± 5.31c

8.84 ± 7.58c

Total Fungal Count (CFU / ml) × 105

7.67 ± 0.44a

2.83 ± 1.36d

2.00 ± 0.00e

2.50 ± 0.00e

Table 7: Microbial load in the intestine of C. gariepinus fed Z. officinale root-powder (Mean ± SEM).
Means along the same row with same letter are not different (p > 0.05).

Morphological characteristics of bacteria colonies isolates

The cultural and colonial morphology of bacteria found in the intestine of fish fed varying level of Z. officinale root-powder are shown in tables 8 and 9.

Isolates Details

A1

B1

C1

D1

Colour

Creamy

Dull Cream

Dull Cream

White

Shape / Margin

Irregular

Entire

Entire

Entire

Arrangement

Cocci in long chain

Baccilli

Bacilli

Cocci in pair

Surface Appearance

Smooth and Glistening

Smooth

Smooth

Smooth and Glistening

Elevation

Raised

Raised

Raised

Raised

Texture

Dry

Moist

Moist

Dry

Opacity

Translucent

Translucent

Translucent

Translucent

Table 8: Morphological characters of bacteria colonies isolates of C. gariepinus fed Z. officinale root-powder diets (1st replicate)
Key:
A = Treatment one (Control)
B = Treatment five (1 % of Z. officinale root-powder)
C= Treatment six (2 % of Z. officinale root-powder)
D = Treatment seven (3 % of Z. officinale root-powder)
 

Isolates Details

A2

B2

C2

D2

Colour

Creamy

Creamy

Creamy

White

Shape / Margin

Irregular

Entire

Entire

Entire

Arrangement

Cocci in chain

Baccilli

Baccilli

Cocci in pair

Surface Appearance

Smooth and Glisteningv

Smooth and Glistening

Smooth and Glistening

Smooth and Glistening

Elevation

Raised

Raised

Raised

Raised

Texture

Mucoid

Moist

Moist

Dry

Opacity

Translucent

Translucent

Translucent

Translucent

Table 9: Morphological characters of bacteria colonies isolates of C. gariepinus fed Z. officinale root-powder diets (2nd replicate)

Biochemical characterization of the bacteria isolate

Table 10 shows the biochemical test of the bacteria isolated from the intestine of the fish fed Z. officinaleroot-powder and the control showing the occurrence of the bacteria in the fish intestine.

Isolate

Gram

Spores

Motility

Catalase

Starch hydrolysis

Gelatin liquefaction

Oxidase

Mannitol

Sucrose

Lactose

Glucose

Suspected organism

A1

+

-

-

-

-

-

-

-

+

-

+

Streptococcus agalactiae

A2

+

-

-

-

-

-

-

-

+

-

+

S. agalactiae

B1

+

+

+

+

+

+

+

+

-

-

+

Baccillus subtilis

B2

+

+

+

+

+

+

+

+

-

-

+

B. subtilis

C1

+

+

+

+

+

+

+

+

-

-

+

B. subtilis

C2

+

+

+

+

+

+

+

+

-

-

+

B. subtilis

D1

+

-

-

-

+

-

-

+

-

+

+

E. faecialis

D2

+

-

-

-

+

-

-

+

-

+

+

E. faecialis

Table 10: Biochemical test of the Bacteria isolates
Key: +: Positive,-: Negative

Colonial characteristics of fungal isolate

The morphological characters of fungal isolated from the intestine of C. gariepinus are presented in tables 11 and 12.

Treatment

Colour of spore

Appearance of mycelia

Type of spores

Arrangement of spores

Shape of spore

Type of hyphae

Identified organism

A11

Black

Grainy

Conidiosphore

In masses

Cylindrical

Septate

Aspergillus niger

A12

Yellow black

Fluffy

Sporangiospore

In masses

Globuse

Aseptate

Mucor mucedo

B1

Black

Grainy

Conidiosphore

In masses

Cylindrical

Septate

A. niger

C1

Yellow black

Fluffy

Sporangiospore

In masses

Globuse

aseptate

Mucor mucedo

D1

Black

Grainy

Conidiosphore

In masses

Cylindrical

septate

A. niger

Table 11: Fugal Isolates from fish Intestine (1st replicate)
 

Treatment

Colour of spore

Appearance of mycelia

Type of Spores

Arrangement of spores

Shape of spore

Type of hyphae

Identified organism

A21

Black

Grainy

Conidiosphore

In masses

Cylindrical

Septate

A. niger

A22

Black

Grainy

Conidiosphore

In masses

Cylindrical

Septate

A. niger

B2

Black

Grainy

Conidiosphore

In masses

Cylindrical

Septate

A. niger

C2

Black

Grainy

Conidiosphore

In masses

Cylindrical

Septate

A. niger

D2

Black

Grainy

Conidiosphore

In masses

Cylindrical

Septate

A. niger

Table 12: Fungal isolates from fish intestine (2nd replicate)

DISCUSSION

The values of the physico-chemical parameters observed in the experimental tanks during this study were within the range recommended for C. gariepinus [22,23]. The achievement of this was as a result of optimum water management practices.

There was a general increase in weight gain with the highest growth performance observed in fish fed 1 % and 3 % Z. officinale root-powder. Fish fed 1 % Z. officinale root-powder diet recorded the highest and showing best growth responses in terms of Percentage Weight Gained (PWG), Specific Growth Rate (SGR) and Apparent Net Protein Utilization (ANPU). The fish fed the control diet recorded the lowest values of PWG and ANPU. This is in agreement with the work of Bello et al. [24], who recorded similar increase in weight gain of fish when fed diets supplemented with Walnut leaf and Onion bulb residues. There was a rapid growth rate of C. gariepinus in the first few weeks of culture in the study; this could probably due to initial starvation of the fish which made them more metabolically active. This is similar to report of Obasa and Faturoti [25] who recorded similar observation in the growth of juvenile Heterotis niloticus. They recorded an increase in growth of the fish as they were subjected to delay in feed distribution.

The remarkable performances of fish fed the supplemented diets in PWG, SGR and ANPU over control diet could be due to the presence of growth promoters: zingiberene, glycosides and terpenoids in 1 % Z. officinale root-powder. This is in agreement with the result of Onibi et al. [26], suggested that ginger and garlic supplements collectively or individually improved growth performance of broilers. This was corroborated by Haghighi and Rohani [13].

There was a reduction in the total feed intake at higher levels of (2 % and 3 %) of Z. officinale root-powder; this could probably due to lower palatability of the two diets as a result of the presence of tannin in the Z. officinale root-powder. The work of Fasasi et al. [12], stated that tannins interfere with digestion by displaying anti-trypsin and anti- amylase activity, forming complexes with vitamin B12 and interfering with the bioavailability of proteins. Also, authors such as Azaza et al. [27], are of opinion that the presence of 2.4 % tannin in faba beans (Vicia faba L. var. minuta) might be responsible for low palatability and consequently low feed intake in Nile tilapia. The increased FCR as revealed in fish fed Z. officinaleroot-powder and higher than the fish fed control diet is similar to the report of Bello et al. [24], who revealed that inclusion of 1.5 % walnut leaf increased FCR in the supplemented groups than the control. This was also corroborated by the work of Abd El - Rahman [28] which indicated that the inclusion of Propolis - ethanolic extract and crude propolis increased the FCR, FER and PER in the supplemented groups when compared with the control. Also the findings of Zomrawi et al. [29], who showed that were no significant differences (p > 0.05) in FCR among all dietary Z. officinale root-powder treatments which corroborated with the result obtained in this present study.

The better SGR recorded in the supplemented diets is in correlation with the result of Abou-Zeid [30] which showed that Allium sativum supplementation positively affected O. niloticus biomass and Specific Growth Rate (SGR).

There is a reduction in survival rate in fish fed the Z. officinale root-powder diets as seen in this experiment could be as a result of some phytochemicals present in the phytobiotic. Fish fed 2 % and 3 % Z. officinale root-powder recorded the highest mortality rate. The findings of Ashade et al. [31], also revealed that the mortality rate of fish fed untreated ginger peel increased with respect to the different concentrations and the highest concentration having more mortality rate.

The bacteria flora of the intestine of C. gariepinus consisted of Bacillus substilis, Streptococcus agalactiaeand Enterococcus faecialis. This could indicate that Z. officinale root-powder favours or promote the growth of gram positive bacteria in the intestine of C. gariepinus. This is in the agreement with the work of Olojo et al. [32] who observed similar bacteria flora in the intestine of C. gariepinus. This is corroborated by Nwabueze [11] who observed similar bacteria flora from the epidermal mucus of C. gariepinus fed ginger powder. Pandy et al. [33], also reported that all medicinal plants are able to stimulate only non-specific immune responses and suggested that the medicinal plants could be used to treat diseases. This gastrointestinal microbes could also be advantageous in that they help in digestive processes of fish such as microbial breakdown of chitin, collagen, cellulose and they may also supply fatty acids and other vitamins to the host and hence promoting growth of the fish [34]. These might have taken place in the fish fed the dietary supplement. The gastrointestinal microbes also prevent colonization of the fish by other microbes that might otherwise be pathogenic. B. substilis has been reported to promote performance and immune responses which might have taken place in fish fed ZOP1 and ZOP3. Tannins and alkaloid are present in ginger roots powder. Tannins and saponins are responsible for antibacterial activity, and able to permeate cells without destroying cell morphology. Tannins inhibit microbial proliferation by denaturation of enzymes of involved in microbial metabolism [35]. This might have occurred in the intestine of fish fed the dietary supplement because majorly the bacteria flora in the intestine of C. gariepinus are mostly gram positive bacteria and thus these phytochemicals probably inhibited the growth of gram negative bacteria and promoted the growth of gram positive bacteria. 

There were differences (p < 0.05) in the reductions in the total bacteria count of fish fed varying levels of Z. officinale root-powder than the control. This is in agreement with the report of Bello et al. [24], who observed that there was a decrease in values of the bacterial load of the supplemented groups (onion bulb and walnut leaves) as the level of inclusion (0.5 %, 1.0 % and 2.0 %) increased and as the months increased. The enterobacteriaceae load in the intestine of C. gariepinus fed Z. officinale root-powder were lower than the control with significant decrease (p < 0.05) as recorded in this study. This decrease in bacteria load in fish as observed in this study has been linked to the presence of antimicrobial properties in Z. officinale root-powder. This study suggests that Z. officinale root-powder is more effective as an antibacterial. Tannins also have shown potential antiviral, antibacterial properties [36]. 

The fungi flora of the intestine of C. gariepinus fed diets containing varying levels of Z. officinale root-powder revealed the presence of Aspergillus niger and Mucor mucedo. A. niger and M. mucedo are associated to food spoilage. Their presence in the fish intestine could indicate that some of the experimental feeds might have been rancid and support the growth of these microbes.

Also, there were differences (p < 0.05) in the reductions in the total fungal count of fish fed diets supplemented with varying levels of Z. officinale root-powder than the control diet. Also, this study indicated that Z. officinale root-powder could act effectively as an antifungal. This is in line with the work of Idris et al. [37], which worked on the effect of different concentration of ginger on smoke-dried C. gariepinus, and found that ginger reduced the free fatty acid values, tri-methylamine values and reduced the fungi load of the processed fish.

CONCLUSION

The results of this present study revealed that dietary supplementation of Z. officinale root-powder for C. gariepinus fingerlings are encouraged to improve the growth performance; nutrient utilization and gastrointestinal microbes of C. gariepinus fingerlings. This could be attributed to the growth promoting activity and immunostimulation properties of the dietary supplement. Inclusion level of 1 % Z. officinaleroot-powder in the diet of fish is best considered because of its good FCR, high ANPU, SGR and weight gain. Based on this present study, inclusion of 2 % Z. officinale root-powder is relatively considered to act as an antibacterial and antifungal in the fish and improving the gastrointestinal microbes of C. gariepinusfingerlings.

AUTHORS’ CONTRIBUTIONS

The main author Mrs. Adegbesan S.I. of this work centers on carrying out this research in the field and manuscript preparation. The co-author, Prof. S.O. Obasa worked on manuscript proof reading and offering supervisory role for the conduct of this experiment. The area of gastro-intestinal microbes of this research was supervised by the second co-author, Prof. A.K. Akintokun a microbiologist. The third co-author, Dr. Abdulraheem I. assisted in the supervision of the aquaculture aspect of the research.

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Citation: Adegbesan SI, Obasa SO, Akintokun AK, Abdulraheem I (2019) Effects of Dietary Supplementation of Zingiber officinale Root-Powder on Growth, Nutrient Utilization and Intestinal Microbes of African Mud Catfish (Clarias gariepinus) Fingerlings. J Aquac Fisheries 3: 016.

Copyright: © 2019  Sherifat Ibidunni Adegbesan, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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