Journal of Stem Cells Research Development & Therapy Category: Medical Type: Short Commentary

Stem Cells Administration in Cystic Kidney Animal Models: New Potential Therapeutic Approach

Daniela Nardozi1* and Norbert Gretz1
1 Medical research center, Medical Faculty Mannheim, University of Heidelberg, Theodor-Kutzer-Ufer, Mannheim, Germany

*Corresponding Author(s):
Daniela Nardozi
Medical Research Center, Medical Faculty Mannheim, University Of Heidelberg, Theodor-Kutzer-Ufer, Mannheim, Germany

Received Date: Jun 08, 2022
Accepted Date: Jun 16, 2022
Published Date: Jun 23, 2022


Cystic Kidney Disease (CKD) represents a heterogeneous group of chronic disorders and the fourth leading cause of end stage renal disease. CKD incidence has been increasing every year for several decades [1,2]. Dialysis and kidney transplantation are often the only efficient treatments available for patients. This is the reason why, nowadays, CKD has a huge human and economic impact on society and healthcare [1,3]. 

In the last years, Mesenchymal Stromal Cell (MSC) has been widely used in preclinical studies as a promising treatment in numerous acute and chronic kidney disease [4]. In fact, the immunomodulatory capability and their anti-inflammatory and anti-apoptotic properties make the MSC a valid therapeutic approach [5-8]. Furthermore, MSC therapy may lead to lower public health costs. Lately, the focus has been set on the factors released by the cells in the media, such as chemokines, cytokines, angiogenic and growth factors and their role as new potential therapeutic factors. 

To date, only two preclinical studies testing the therapeutic effect of allogenic MSC in CKD rat models have been published [9,10]. Both showed a beneficial amelioration of the renal function in PKC rats following MSC treatment. 

In our recent study, we investigated and demonstrated for the first time the potential therapeutic effects of human MSC and their derived conditional media administration in the CKD animal model [11]. Human MSC can escape the host’s immune system due to the immunomodulatory capability. For this reason and in the light of potential clinical application, we tested the therapeutic effect of two different types of human MSC and their derived conditioned media. Our results highlighted an amelioration of kidney function together with outstanding changes in the gene expression profile [11]. MSC’s secreted factors, such a cytokines, could have led the reprogramming of metabolism related pathways together with the calcium signaling pathways and the resulting cyst size reduction. Major changes have been observed also in the immune related pathways, indicating a restoration of the maincellular physiological responses. 

Despite the recent promising results provide the ground for new therapeutic approaches; further studies have to be performed to better understand the MSC and their derived conditioned media mode of action. 

Moreover, even today, one of the major difficulties in planning such an experimental design is the lack of knowledge concerning the suitable administration route. The intravenous (i.v.) injection is so far the most common administration route yet by using this route cells might be trapped in the lungs with the risk of causing emboli [12-15]. To overcome this problem, in our study, we administrated the cell either by i.v. or intraperitoneal (i.p.) injection. We demonstrated that i.p. is safety and efficient alternative route for cell administration. 

In order to achieve the most desired outcome, further studies have to be performed to assess the proper cell dose as well.


  1. Owen WF (2003) Patterns of care for patients with chronic kidney disease in the United States: dying for improvement. J Am Soc Nephrol 14: 76-80.
  2. Bruck K, Stel VS, Gambaro G, Hallan S, Volzke H, et al. (2016) CKD Prevalence Varies across the European General Population. J Am Soc Nephrol 27: 2135-2147.
  3. Cloutier M, Manceur AM, Guerin A, Aigbogun MS, Oberdhan D, et al. (2020) The societal economic burden of autosomal dominant polycystic kidney disease in the United States. BMC Health Serv Res 20: 126-126.
  4. Crigna AT, Daniele C, Gamez C, Balbuena SM, Pastene DO, et al. (2018) Stem/Stromal Cells for Treatment of Kidney Injuries With Focus on Preclinical Models. Front Med (Lausanne) 5: 179-179.
  5. Schatton T, Yang J, Kleffel S, Uehara M, Barthel SR, et al. (2015) ABCB5 Identifies Immunoregulatory Dermal Cells. Cell Rep 12: 1564-1574.
  6. Lee JM, Jung J, Lee HJ, Jeong SJ, Cho KJ, et al. (2012) Comparison of immunomodulatory effects of placenta mesenchymal stem cells with bone marrow and adipose mesenchymal stem cells. Int Immunopharmacol 13: 219-224.
  7. Riedl J, Leonard MP, Eide C, Kluth MA, Ganss C, et al. (2021) ABCB5+ dermal mesenchymal stromal cells with favorable skin homing and local immunomodulation for recessive dystrophic epidermolysis bullosa treatment. Stem Cells 39: 897-903.
  8. Zhu XY, Klomjit N, Conley SM, Ostlie MM, Jordan KL, et al. (2021) Impaired immunomodulatory capacity in adipose tissue-derived mesenchymal stem/stromal cells isolated from obese patients. J Cell Mol Med 25: 9051-9059.
  9. Franchi F, Peterson KM, Xu R, Miller B, Psaltis PJ, et al. (2015) Mesenchymal Stromal Cells Improve Renovascular Function in Polycystic Kidney Disease. Cell Transplant 24: 1687-1698.
  10. Kelly KJ, Zhang J, Han L, Kamocka M, Miller C, et al. (2015) Improved Structure and Function in Autosomal Recessive Polycystic Rat Kidneys with Renal Tubular Cell Therapy. PLoS One 10:0131677.
  11. Nardozi D, Palumbo S, Khan AUM, Sticht C, Bieback K, et al. (2022) Potential Therapeutic Effects of Long-Term Stem Cell Administration: Impact on the Gene Profile and Kidney Function of PKD/Mhm (Cy/+) Rats. J Clin Med 11:2601.
  12. Kurtz A (2008) Mesenchymal stem cell delivery routes and fate. Int J Stem Cells 1: 1-7.
  13. Barbash IM, Chouraqui P, Baron J, Feinberg MS, Etzion S, et al. (2003) Systemic delivery of bone marrow-derived mesenchymal stem cells to the infarcted myocardium: feasibility, cell migration, and body distribution. Circulation 108: 863-868.
  14. Li H, Guo Z, Jiang X, Zhu H, Li X, et al. (2008) Mesenchymal stem cells alter migratory property of T and dendritic cells to delay the development of murine lethal acute graft-versus-host disease. Stem Cells 26: 2531-2541.
  15. Schrepfer S, Deuse T, Reichenspurner H, Fischbein MP, Robbins RC, et al. (2007) Stem cell transplantation: the lung barrier. Transplant Proc 39: 573-576.

Citation: Nardozi D, Gretz N (2022) Stem Cells Administration in Cystic Kidney Animal Models: New Potential Therapeutic Approach. J Stem Cell Res Dev Ther 8: 095.

Copyright: © 2022  Daniela Nardozi, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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