Journal of Stem Cells Research Development & Therapy Category: Medical Type: Short Commentary
The Tgf-B1 Over Expression has Major Impact on Multilineage Differentiation of Mesenchymal Stromal Cells
- Hasan SALKIN1*
- 1 Department Of Medical Services Andtechnique, Programme Of Pathology Laboratory Techniques, Vocational School, Department Of Histology And Embryology, Faculty Of Medicine, Erciyes University, Büyükçekmece/Istanbul 34550, Kayseri, Turkey
*Corresponding Author:
Hasan SALKINDepartment Of Medical Services Andtechnique, Programme Of Pathology Laboratory Techniques, Vocational School, Department Of Histology And Embryology, Faculty Of Medicine, Erciyes University, Büyükçekmece/Istanbul 34550, Kayseri, Turkey
Tel:+90 5301255808,
Fax:+90 2128722830
Email:hasansalkin@beykent.edu.tr
Received Date: Jul 24, 2019 Accepted Date: Aug 01, 2019 Published Date: Aug 08, 2019
Keywords
Mesenchymal Stromal Cells; adipocytes; osteoblasts; chondrocytes
Article
Loss of therapeutic potential would limit their utilization in transplantation medicine. TGF-β1 has been reported to induce senescence in some tumor cells [8,9]. However, whether TGF-β affects senescence of DPSCs has still not been elucidated. Also, the effects on apoptosis, cell cycle and DNA damage of DPSCs of TGF-β1 over expression have not been investigated yet. In this study is to investigate the surface markers, multilineage differentiation, viability, apoptosis, cell cycle, DNA damage and senescence of Human Dental Pulp-Derived Mesenchymal Stromal Cells (hDPSC) which transfected by TGF-β1 gene. TGF-β1 gene transfer into hDPSCs was performed by electroporation method after the plasmid was prepared.
The transfection efficiency was achieved by using western blot and flow cytometry analyses and GFP transfection. Mesenchymal Stem Cell (MSC) markers, multilineage differentiation, cell proliferation, apoptosis, cell cycle, DNA damage and cellular senescence assays were performed by comparing the transfected and non-transfected cells. Strong expression of TGF-β1 in pCMV-TGF-β1-transfected hDPSCs was detected in flow cytometry analysis. TGF-β1 transfection efficiency was measured as 95%. Western blot analysis showed that TGF-β1 protein levels increased at third and sixth days in pCMV-TGF-β1-transfected hDPSCs. The continuous TGF-β1 overe xpression inhDPSCs did not influence the immunophenotype and surface marker expression of MSCs. Our results showed that TGF-β1 increased osteogenic and chondrogenic differentiation, but decreased adipogenic differentiation. Over expression of TGF-β1 increased the proliferation rate and decreased total apoptosis in hDPSCs. The number of cells at “S” phase was higher with TGF-β1 transfection. Cellular senescence decreased in TGF-β1 transfected group.These results reflect that TGF-β1 has major impact on MSC differentiation. TGF-β1 transfection has no effect on cell surface markers. TGF-β1 transfection has positive effects on proliferation, cell cycle and prevents cellular senescence and apoptosis. In further studies, it will be essential to determine whether TGF-β1 can play a role in attempts to use MSC for therapeutic approaches. With this study, cells were produced with increased differentiation potentials and strengthened biological features that may be used in regenerative medicine, tissue engineering, gene therapy and cellular therapy studies (Figure 1).

A) Chondrogenic differentiation and safranin-ostaining.
B) Graphic show adipored assay fluorimetric measurement results for adipogenic differentiation.
C) Microscope magnifications are 4×.Scale bar is 100 μm. *p<0.05.
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Citation:SALKIN H (2019) The Tgf-β1 Over Expression has Major Impact on Multilineage Differentiation of Mesenchymal Stromal Cells. J Stem Cell Res Dev Ther 5: 013.
Copyright: © 2019 Hasan SALKIN, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.